Prenatal diagnosis of neonatal alloimmune thrombocytopenia using allele- specific oligonucleotide probes

Prenatal diagnosis of neonatal alloimmune thrombocytopenia using allele-specific oligonucleotide probes.

The prediction of neonatal alloimmune thrombocytopenia (NATP) in affected families has, in the past, been based on information about gene frequencies of the antigen systems involved, parental phenotyping, and fetal platelet counts. We explored the feasibility of allele-specific oligonucleotide probe typing for PIA antigens to determine the risk of second or subsequent fetuses in families where ...

Prenatal diagnosis of hemoglobinopathies by using polymerase chain reaction and allele-specific oligonucleotide probes.

The prevalence of hemoglobinopathies, their widespread distribution, and their potential for clinical severity make prenatal diagnosis and testing for carriers of these disorders important public health issues. Although current hematologic tests are adequate for carrier screening, pre-natal diagnosis during the first trimester is possible only by using molecular pathology techniques. Direct ana...

Fetal and neonatal alloimmune thrombocytopenia: prenatal interventions.

Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is a potentially devastating condition, which may lead to intracranial haemorrhage (ICH) in the fetus or neonate, often with death or major neurological damage as consequence. In the absence of screening, preventive measures are only possible in the next pregnancy of women with an affected child. Controversy exists on the best intervention ...

Neonatal alloimmune thrombocytopenia.

Neonatal alloimmune thrombocytopenia: current considerations.

Some mothers produce antibodies to the platelet antigens of their fetuses. Exposure to these antigens may occur owing to prior transfusions or through feto-maternal hemorrhage during gestation or delivery. The sensitizing antigen is usually an epitope of one of the glycoproteins (GP) found on the platelet membrane. Specific GPs act as receptors for factors important in hemostasis, such as von W...